mouse seng kit  (R&D Systems)

Journal: Journal of Clinical Medicine

Article Title: Molecular and Functional Cargo of Plasma-Derived Exosomes in Patients with Hereditary Hemorrhagic Telangiectasia

doi: 10.3390/jcm13185430

Figure Lengend Snippet: Antibodies for Western blot.

Article Snippet: Soluble Endoglin , Santa Cruz (Santa Cruz, CA, USA), SC-20072 , 1:200 in 5% BSA , Mouse , 61 kDa , Reducing , PVDF.

Techniques: Western Blot, Membrane

Journal: Journal of Clinical Medicine

Article Title: Molecular and Functional Cargo of Plasma-Derived Exosomes in Patients with Hereditary Hemorrhagic Telangiectasia

doi: 10.3390/jcm13185430

Figure Lengend Snippet: Soluble Endoglin levels in HD and HHT exosomes. ( A ) Representative Western blot of HD and HHT exosomes for the angiogenesis-related protein sENG and the exosomal marker TSG101. Numbers below lanes indicate band intensities of sENG normalized to TSG101 using lane normalization factors. ( B ) Normalized sENG values of HD and HHT exosomes (n = 17). Age- and gender-matched pairs of HDs and HHT patients whose plasma was used for exosome isolation are connected by a line. ( C ) Normalized sENG values of HHT exosomes (n = 17) were correlated to total exosomal protein levels, as determined by Bicinchoninic acid (BCA) assay. Spearman’s rank correlation coefficient (r) and correlation significance (p) were calculated. ( D ) Normalized sENG values of HD exosomes (n = 17) were correlated to the total exosomal protein levels, as determined by BCA assay. Pearson coefficient of correlation (R2) and correlation significance (p) are shown.

Article Snippet: Soluble Endoglin , Santa Cruz (Santa Cruz, CA, USA), SC-20072 , 1:200 in 5% BSA , Mouse , 61 kDa , Reducing , PVDF.

Techniques: Western Blot, Marker, Clinical Proteomics, Isolation, BIA-KA